| |
Ex / Em (nm) |
minimum
scale
guaranteed O.D |
corresponding grade |
supplement |
| GEL filtration |
OPC |
HPLC |
| Acridinium |
|
hv 430 nm |
2 O.D. |
– |
– |
● |
|
| Azide |
5'azide |
– |
2 O.D. |
– |
– |
● |
|
| 3'azide |
– |
2 O.D. |
– |
– |
● |
| Alkyne
(Hexynyl Modifier) |
– |
2 O.D. |
– |
– |
● |
|
| amino |
5'amino |
– |
2 O.D. |
– |
– |
● |
Modification of primary amine. It's possible to provide you with internal modification which is introduced via linker at the base site of base sequences. In case of modification at 5' terminal, there are C6 type and C12 type depending on length of linkers. |
|
– |
3 O.D. |
● |
– |
– |
| 3'amino |
– |
2 O.D. |
– |
● |
● |
internal amino
(dA–amino) |
– |
2 O.D. |
– |
– |
● |
internal amino
(dG–amino) |
– |
2 O.D. |
– |
– |
● |
internal amino
(dC–amino) |
– |
2 O.D. |
– |
– |
● |
internal amino
(dT–amino) |
– |
2 O.D. |
– |
– |
● |
| ATTO
series |
|
– |
2 O.D.*3 |
– |
– |
● |
Fluorescent materials from ATTO-TEC Co. They line up dyes of various wavelengths as fluorescent materials for NGRL's double Labeled probes using license free materials. Please refer to Double Labeled probes. |
| biotin |
5'biotin |
– |
2 O.D. |
– |
– |
● |
3'biotin is TEG linker. Others are C6 linkers. |
3'biotin
(TEG-linker) |
– |
2 O.D. |
– |
– |
● |
internal biotin
(biotin–dA) |
– |
2 O.D. |
– |
– |
● |
internal biotin
(biotin–dG) |
– |
2 O.D. |
– |
– |
● |
internal biotin
(biotin–dC) |
– |
2 O.D. |
– |
– |
● |
internal biotin
(biotin–dT) |
– |
2 O.D. |
– |
– |
● |
| 5' biotin-TEG |
– |
2 O.D. |
– |
– |
● |
According to specifications of TEG linker ( 15 atoms) of 5' biotin. |
| imino-biotin |
– |
2 O.D. |
– |
– |
● |
Iminobiotin changes its affinity against streptavidin by depending on PH. It has high affinity in alkali condition and it decreases in acid condition. |
| BHQ® Series |
3'BHQ-0® |
QR=430-520nm |
2 O.D. *3 |
– |
– |
● |
Dark quencher from Biosearch Technologies. They line up 4 kinds of quenchers of different quench range. There is a usage to modify BHQ at 3' terminal and fluorescent materials at 5' terminal and make it as a hydrolyzed probe. |
| 3'BHQ-1® |
QR=480-580nm |
2 O.D. *3 |
– |
– |
● |
| 3'BHQ-2® |
QR=560-670nm |
2 O.D. *3 |
– |
– |
● |
| 3'BHQ-3® |
QR=620-730nm |
2 O.D. *3 |
– |
– |
● |
| 8-Br-dA |
|
– |
2 O.D. |
– |
– |
● |
A modification in which Br is introduced in one part of nucleotide. It is used for research for crystallographic structure. And, it is also used for crosslink experiments for research of protein·DNA complex structure by making use of light instability. |
| 5-Br-dC |
|
– |
2 O.D. |
– |
– |
● |
| 8-Br-dG |
|
– |
2 O.D. |
– |
– |
● |
| 5-Br-dU |
|
– |
2 O.D. |
– |
– |
● |
| Cy®-dyes |
5'Cy®-3 |
552 / 565 |
2 O.D. |
– |
– |
● |
|
| 5'Cy®-3.5 |
581 / 596 |
2 O.D. |
– |
– |
● |
| 5'Cy®-5 |
650 / 667 |
2 O.D. |
– |
– |
● |
| 5'Cy®-5.5 |
678 / 703 |
2 O.D. |
– |
– |
● |
| Dabsyl |
3'Dabsyl |
– |
1 O.D. |
– |
– |
● |
It's a collisional quenching type quencher which causes quenching effect when it comes to closest point to fluorescent materials. |
| Dabcyl |
3'Dabcyl |
– |
1 O.D. |
– |
– |
● |
| DIG |
5'DIG |
– |
2 O.D. |
– |
– |
● |
It is used for probes for experiments making use of hybridizations such as in situ hybridization and northern blotting. DIG-labeled DNA Oligos is detectable by fluorescence-labeled anti-DIG antibody. In addition to modifications at 5' terminal and 3' terminal, it is possible to undergo internal modification which is introduced into base site of base sequences. Also, it is possible to select DIG-tailing with which multiple DIG-dUTP are bound to 3' terminal of synthesized DNA Oligos by enzymatic reaction. |
| 3'DIG |
– |
2 O.D. |
– |
– |
● |
| internal DIG (DIG-dA) |
– |
2 O.D. |
– |
– |
● |
| internal DIG (DIG-dG) |
– |
2 O.D. |
– |
– |
● |
| internal DIG (DIG-dC) |
– |
2 O.D. |
– |
– |
● |
| internal DIG (DIG-dT) |
– |
2 O.D. |
– |
– |
● |
| 3'DIG tailing 100pmole |
– |
100pmole*1 |
– |
● |
● |
| 3'DIG tailing 1000pmole |
– |
1000pmole*1 |
– |
● |
● |
| DNP |
5'DNP |
– |
2 O.D. |
– |
– |
● |
DNP is modified at 5' terminal. DNP-modified DNA Oligos is detectable by DNP antibody.
|
| d-spacer |
|
– |
2 O.D. |
– |
● |
● |
It is used when inserting only deoxyribose link having no base site into DNA chains |
| dU |
|
– |
2 O.D. |
– |
● |
● |
|
| 5-FAM |
5'5-FAM |
493 / 522 |
2 O.D. |
– |
– |
● |
Isomer of 6-FAM. |
| 6-FAM |
5'6-FAM |
494 / 525 |
2 O.D. *3 |
– |
– |
● |
One of the most frequently used fluorescent materials in real-time PCR. Basic structure part is same as FITC. |
| Ferrocene |
5'Ferrocene
(short linker-type) |
– |
– |
– |
– |
● |
|
5'Ferrocene
(long linker-type) |
– |
– |
– |
– |
● |
| FITC |
5'FITC |
494 / 518 |
2 O.D. |
– |
– |
● |
|
| 3'FITC 1nmole |
494 / 518 |
1nmole *2 |
– |
– |
● |
|
| 3'FITC 3nmole |
494 / 518 |
3nmole *2 |
– |
– |
● |
| 3'FITC 10nmole |
494 / 518 |
10nmole *2 |
– |
– |
● |
internal FITC
(FITC-dA) |
494 / 518 |
2 O.D. |
– |
– |
● |
|
internal FITC
(FITC-dG) |
494 / 518 |
2 O.D. |
– |
– |
● |
internal FITC
(FITC-dC) |
494 / 518 |
2 O.D. |
– |
– |
● |
internal FITC
(FITC-dT) |
494 / 518 |
2 O.D. |
– |
– |
● |
| HEX |
5'HEX |
535 / 556 |
2 O.D. *3 |
– |
– |
● |
|
| 5-I-dU |
|
– |
2 O.D. |
– |
– |
● |
A modification in which iodine(I) is introduced into one part of nucleotide. It is used for investigation of crystallographic structure of nucleotide and also used for crosslink experiments of protein·DNA complex structure by making use of light instability. |
| inosine |
|
– |
2 O.D. |
– |
● |
● |
|
| |
– |
3 O.D. |
● |
– |
– |
| LightCycler®Red |
5'LightCycler®Red640
(3'P)1nmole |
625 / 640 |
1nmole*2 |
– |
– |
● |
|
| 5'LightCycler®Red640(3'P)3nmole |
625 / 640 |
3nmole*2 |
– |
– |
● |
| 5'LightCycler®Red640(3'P)10nmole |
625 / 640 |
10nmole*2 |
– |
– |
● |
| 5'LightCycler®Red705(3'P)1nmole |
685 / 705 |
1nmole*2 |
– |
– |
● |
| 5'LightCycler®Red705(3'P)3nmole |
685 / 705 |
3nmole*2 |
– |
– |
● |
| 5'LightCycler®Red705(3'P)10nmole |
685 / 705 |
10nmole*2 |
– |
– |
● |
| 5'LightCycler®Red610(3'P)1nmole |
590 / 610 |
1nmole*2 |
– |
– |
● |
| 5'LightCycler®Red610(3'P)3nmole |
590 / 610 |
3nmole*2 |
– |
– |
● |
| 5'LightCycler®Red610(3'P)10nmole |
590 / 610 |
10nmole*2 |
– |
– |
● |
| 5-Me-dC |
|
– |
2 O.D. |
– |
– |
● |
Modified base of methylated position 5 of deoxycytidine can be introduced. It is reported that Tm value increased by 1.3℃ by introduction of this reagent. |
| 5-Nitroindole |
|
– |
2 O.D. |
– |
– |
● |
Kinds of modified bases which show degenerating site effect at the time of forming double-stranded chains of DNA. |
| 3-Nitropyrrole |
|
– |
2 O.D. |
– |
– |
● |
| N6-Me-dA |
|
– |
2 O.D. |
– |
– |
● |
It is used when 6th position of nitrogen atom of deoxyadenosine introduces methylated modification. |
| 5-OH-dC |
|
– |
2 O.D. |
– |
– |
● |
It is used when introducing modified base in which hydroxyl group bound at positions 5 of cytosine and uracil. |
| 5-OH-dU |
|
– |
2 O.D. |
– |
– |
● |
| O6-Me-dG |
|
– |
2 O.D. |
– |
– |
● |
It is used when 6th position of oxygen atom of guanosine introduces methylated modification. |
| 8-OMe-dG |
|
– |
2 O.D. |
– |
– |
● |
It is used when introducing modified base of 8th position of guanosine which is turned to OMe. |
| 8-oxo-dA |
|
– |
2 O.D. |
– |
– |
● |
It is used when introducing modified bases in which 8th positions of adenosine and guanine are oxidized respectively. |
| 8-oxo-dG |
|
– |
2 O.D. |
– |
– |
● |
| Phosphorylation |
5'Phosphorylation
| – |
2 O.D. |
– |
– |
● |
|
| – |
3 O.D. |
● |
– |
– |
| 3'Phosphorylation |
– |
2 O.D. *2 |
– |
● |
● |
| s-oligo |
s-oligo |
– |
10 O.D. |
– |
– |
● |
|
| s-oligos chimera |
– |
10 O.D. |
– |
– |
● |
|
| SpacerC3 |
|
– |
2 O.D. |
– |
● |
● |
It is used when introducing spacer into phosphodiester bond structure of DNA chains. Spacer C3 will become spacer arm of 3 carbon atoms. It is also possible to introduce "Spacer C12" made of 12 carbon atoms, "Spacer 9" made of 9 atoms spacer of linker type and “ Spacer18” made of 18 atoms spacer.
|
Sulfo-rhodamine 101
(Texas-Red) |
5'Sulfo-rhodamie 101 |
595 / 615 |
2 O.D. |
– |
– |
● |
|
| 3'Sulfo-rhodamine 101 |
595 / 615 |
2 O.D. |
– |
– |
● |
| TAMRA |
5'TAMRA |
555 / 580 |
2 O.D. |
– |
– |
● |
|
| 3'TAMRA |
555 / 580 |
2 O.D. *3 |
– |
– |
● |
| internal TAMRA (TAMRA–dA) |
555 / 580 |
2 O.D. |
– |
– |
● |
| internal TAMRA (TAMRA–dG) |
555 / 580 |
2 O.D. |
– |
– |
● |
| internal TAMRA (TAMRA–dC) |
555 / 580 |
2 O.D. |
– |
– |
● |
| internal TAMRA (TAMRA–dT) |
555 / 580 |
2 O.D. |
– |
– |
● |
| TET |
5'TET |
519 / 539 |
2 O.D. |
– |
– |
● |
|
| thiol |
5'thiol |
– |
2 O.D. |
– |
– |
● |
It is possible to use it for binding reaction with Maleimide structures. It is also used for binding with Cystein residue of protein. |
| 3'thiol |
– |
2 O.D. |
– |
– |
● |
