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| How to send samples |
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| RNA Extraction · DNA Extraction · Synthesis of cDNA · Preparation of Expression Vector |
| Kinds of Samples | How to send samples |
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| cDNA | Please send samples by cooled delivery or packed with cooling agent by home delivery service. |
| genomicDNA |
| Paraffin Segment |
| TissueECultured Cells (RNAlater Treatment Agent) |
| Blood (for DNA Extraction) |
| Primer |
| RNA | Please send samples by frozen delivery or packed with dry ice by home delivery service.
* Please send tissues and cultured cells by frozen delivery packed with dry ice. |
| mRNA |
| TissueECultured Cells (No treatment with RNAlater) |
| Blood/Bone Marrow( for DNA Extraction) |
| Probe * Light Shielding |
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| Pretreatment of tissue samples |
| Sample Type | Pretreatment |
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| Tissue | Treated with preservative agent RNAlater
In case sample is stored at less than -45℃, please sent it in frozen state untreated with RNAlater. |
| Blood/Bone Marrow | After hemolysis treatment with hemolysis buffer, please send the sample after cleansing with PBS and treatment of pellet by preservative agent, RNAlater. |
| Cultured Cell | Please send the sample after cleansing with PBS and treatment of pellet by preservative agent, RNAlater. |
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| * Please use preservative agent of RNAlater(TaKaRa Cat.No.AB001). |
| Please see the attached manual for details of treatment. |
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| Storage temperature and storage period of samples for possible analysis. |
| Sample Type | Storage Temperature | Storage Period |
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| Tissue, Bone Marrow/Blood, Cultured Cells | If treated with RNAlater | -20℃ | storage period should be within 6 months |
| If untreated with RNAlater | under -45℃ | strage period should be within 6 months |
| Paraffin Segment | 4℃(it's also OK in room temperature) | - |
| TotalRNA, mRNA | under -45℃ | strage period should be wintin 6 months |
| cDNA | -20℃ | strage period should be wintin 6 months |
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| Precautions |
| If samples received is not in good condition, please be reminded that we might ask you to send it again.
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