| About Sending Samples |
- Please undergo purification without fail to get rids of PCR products, for desalination and elimination of extra primers and dNTP.
- We recommend plasmid DNA prepared by commercial plasmid purification kit.
(We will not recommend simple purification method such as boil method due to possibility of degradation of quality of analysis data.)
- Please confirm in advance by electrophoresis and so on that there is no impurity such as residual RNA and Genome DNA.
- We accept purification of PCR products as option service.
- Please use PCR grade water for adjustment of volume.
- Please fill in 0.5ml of sample into Eppendorf tube and write "Tube No." of "Application Form of Contracted Service" on the cap of the tube.
- In case you are asking us to undergo synthesis of primers, please declare so in the application form.
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| Please send sample in room temperature. |
| Mailing sample volume |
| Basic α Course
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| Sample | per 1 reaction | 3µg |
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| Primer | per 1 reaction | 6.4pmol/µL | |
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· Please prepare needed volume of samples taking into consideration of volume per reaction.
· Please prepare needed unlabeled primers in case you are going to send primers.
· Please consult us if it's difficult to adjust preparation of above samples.
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| Advance Course |
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| Sample | length of less than 1kbp | more than 4µg/1sample; |
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| length of less than 2kbp | more than 8µg/1sample |
| length of less than 3kbp | more than 12µg/1sample |
| Primer | more than 10µL of 6.4pmol | |
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· Please prepare needed volume of samples and unlabeled primers taking into consideration of volume per reaction.
· Please consult us if other than the above case or if it's difficult to adjust sample preparation.
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| How to browse waveform file |
Waveform file is the file of SCF format.
You can refer to the file by installing following free software.
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Chromas Lite for Windows
It's a free version of charged "Chromas Pro" and "Chromas". Please select "Chromas Lite" from the site.
http://www.technelysium.com.au/chromas_lite.html
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4peaks for Max OSX
It's a software for Mac that acquired "Apple Award" in 2004.
http://mekentosj.com/4peaks/
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FinchTV for Windows, Mac, Linux and Solaris
It's a software usable for Windows, Mac, Linux and Solaris.
http://www.geospiza.com/finchtv/
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