Information on the Tm value: |
oligos <18 bases calculates Tm = 4℃ x (number of G's + C's in the primer) + 2℃ x (number of A's + T's in the primer) . oligos ≧18 bases calculates . Tm =((ΔH * 1000 / (ΔS - 26.26)) - 273.15 - 21.5971) (CONC. 50mM-salt, 0.5µM-primer) |
塩濃度・プライマー濃度は一般的に用いられるPCR条件に設定しています。 |
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